Regulatory

Part:BBa_I12047:Design

Designed by: ryhsiao   Group: Antiquity   (2004-08-23)


BL oscillator, cooperativity + replaced -10 region (Llac), reporter protein, kickstart


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is cooperativity for 434 cI in this oscillator. A protein EYFP (with degradation tag) coding region is placed directly after the lambda cI coding region in order to keep track of the lambda cI levels. This part also includes a "kickstart" that will yield lambda cI if ATC is added. (Differs from BBa_I12045 due to the swapped -10 region of the Lambda cI (LLac))


Source

References